Pancytokeratins

Pankeratin cocktail (Bio Tek Solutions, Santa Barbara, CA, USA) contains monoclonal antibodies AE1, AE3, Cam5.2 and 35bH11. It reacts with virtually all epithelia and their tumours1.
Clone LP34 (Dako Corp, Carpenteria, CA, USA) is a broad spectrum monoclonal antibody reactive with cytokeratins 5, 6, 8, 17 and 19. It reacts with both simple glandular and stratified squamous epithelia1.

It is strongly positive in basal cells of benign prostatic acini, intermediate in PIN and negative in prostatic adenocarcinoma: weak positive staining of luminal cells leads to difficulty of interpretation in lobular atrophy and may cause confusion with the rare cases of adenocarcinoma that are positive for high molecular weight cytokeratins. It is probably most helpful in the differentiation of adenocarcinoma from PIN, while CK5/6 is superior in distinguishing adenocarcinoma from lobular atrophy2.

Pan-cytokeratin (Novocastra, Burlingame, CA, USA) is a cocktail of monoclonal antibodies against cytokeratins 5, 6, 8 and 18. It reacts with most epithelia and their tumours1.
Wide-spectrum screening antibody (Dako) is a rabbit anti-cow polyclonal antibody that reacts with human keratins1.
AE1/AE3
34bE12
KL-1: in kidney, stains only collecting ducts3.

References

1Chu, P. G. and L. M. Weiss (2002). "Keratin expression in human tissues and neoplasms." Histopathology 40(5): 403-39.

2Freeman, A., K. Treurnicht, et al. (2002). "A comparison of basal cell markers used in the prostate." Histopathology 40(5): 492-4.

3Pan Histopathology 2004;45:452 **********

This page last revised 10.1.2005.